INVESTIGADORES
WUILLOUD Rodolfo German
artículos
Título:
HER2 and B-catenin protein location: importance in the prognosis of breast cancer patients and their correlation when breast cancer cells suffer stressful situations
Autor/es:
CUELLO-CARRIÓN, F. DARÍO; SHORTREDE, JORGE; ALVAREZ-OLMEDO, DAIANA; CAYADO-GUTIÉRREZ, NIUBYS; CASTRO, GISELA; ZOPPINO, FELIPE; GUERRERO, MARTIN; MARTINIS, ESTEFANIA; WUILLOUD, RODOLFO; GÓMEZ, NIDIA; BIAGGIO, VERONICA; OROZCO, JAVIER; GAGO, FRANCISCO; CIOCCA, LEONARDO; FANELLI, MARIEL; CIOCCA, DANIEL
Revista:
CLINICAL & EXPERIMENTAL METASTASIS
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2015 vol. 32 p. 151 - 168
ISSN:
0262-0898
Resumen:
In human breast cancer, b-catenin localization has been related with disease prognosis. Since HER2-positive patients are an important subgroup, and that in breast cancer cells a direct interaction of b-catenin/HER2 has been reported, in the present study we have explored whether b-catenin location is related with the disease survival. The study was performed in a tumor bank from patients (n = 140) that did not receive specific anti-HER2 therapy. The proteins were detected by immunohistochemistry in serial sections, 47 (33.5 %) patients were HER2-positive with a long follow-up. HER2-positive patients that displayed b-catenin at the plasma membrane (completely surrounding the tumour cells) showed a significant better disease-free survival and overall survival than the patients showing the protein on other locations. Then we explored the dynamics of the co-expression of b-catenin and HER2 in human MCF-7 and SKBR3 cells exposed to different stressful situations. In untreated conditions MCF-7 and SKBR3 cells showed very different b-catenin localization. In MCF-7 cells, cadmium administration caused a striking change in b-catenin localization driving it from plasma membrane to cytoplasmic and perinuclear areas and HER2 showed a similar localization patterns. The changes induced by cadmium were compared with heat shock, H2O2 and tamoxifen treatments. In conclusion, this study shows the dynamical associations of HER2 and b-catenin and their changes in subcellular localizations driven by stressful situations. In addition, we report for the first time the correlation between plasma membrane associated b-catenin in HER2-positive breast cancer and survival outcome, and the importance of the protein localization in breast cancer samples.