Administration of Lactobacillus plantarum Lp62 to dam rats at the end of delivery and during lactation affects TGF-β1 level and nutritional milk composition, and body weight of pups

MESSIAS, GLADISTONE CORREIA; ROCHA, ANA MARTA NOVAIS; SANTOS, BEATRIZ MANUELA SILVA; BOTELHO, ANA MARQUES; SILVA, DHAÍSA CRISTHINA ALVES; PORTO, ERIKA SANTOS; DOS ANJOS, MARINA LIMA; SOUSA, RAYRA ALMEIDA; SILVA, MARA VIANA; DA ROCHA, THAINARA BARROS; DE MELO, ARACELY VIEIRA; CARNEIRO, MANOELA RIOS TRINDADE; AGUIAR, NAYONARA SANTANA; SANTOS, PATRÍCIA PRADO; DE SOUZA, ERIKA PEREIRA; CRUZ, MARILUZE PEIXOTO; MARQUES, LUCAS MIRANDA; REZENDE, RAQUEL PASSOS; ROMANO, CARLA CRISTINA; UETANABARO, ANA PAULA; VINDEROLA, GABRIEL; YATSUDA, REGIANE

EUROPEAN JOURNAL OF NUTRITION

DR DIETRICH STEINKOPFF VERLAG

Año: 2018

1436-6207

Purpose: Lactobacillus plantarum Lp62 is a lactic acid bacteria strain that has been isolated from cocoa beans and exhibited probiotic potential. The influence of oral administration of L. plantarum Lp62 on the growth of rat?s pups; on yield, cytokines and milk composition was studied. Methods: Lactobacillus plantarum Lp62 is a lactic acid bacteria strain that has been isolated from cocoa beans. It was administered daily by gavage to Wistar rats (n = 8), from the 7th day before delivery and for 20 days during lactation, in a concentration of 1.44 × 109 CFU/rat. The dam and pups were weighed and milk was collected at 12th and 19th day for determination of protein, triglycerides, cholesterol and lactose by colorimetric assays. TGF-β1 milk levels were analyzed by ELISA. The mammary glands of rats were removed for histological analysis. To detect statistical differences between the groups, tests of mean differences at a significance level of 5% was performed. Results: Supplementation with L. plantarum L62 resulted in significant higher weight of pups (p < 0.05), with similar weight on dams (p > 0.05). The milk yield was not altered by L. plantarum treatment, but the levels of protein, triglycerides and cholesterol were increased (p < 0.05), with no difference in lactose concentration (p > 0.05). Levels of TGF-β1 were higher in the milk of L. plantarum treatment (p < 0.05). Conclusions: The treatment of dams at the end of pregnancy and lactation with L. plantarum Lp62 increased nutritional content of milk, probably contributing to the higher weight of the pups. The higher levels of TGF-β1 in the milk, could promote immune benefits to the pups. Further studies in this field are needed to prove the potential use of L. plantarum Lp62 as a probiotic.