INVESTIGADORES
VINDEROLA Celso Gabriel
artículos
Título:
Selection of Lactic Acid Bacteria with Probiotic Potential Isolated from the Fermentation Process of “Cupuaçu” (Theobroma grandiflorum)
Autor/es:
ORNELLAS, ROBERTA MARIA SANTOS; SANTOS, TIZA TELES; ARCUCIO, LEONARDO BORGES; SANDES, SÁVIO HENRIQUE CICCO; OLIVEIRA, MAYARA MESSIAS; DIAS, CRISTIANO VILLELA; DE CARVALHO SILVA, SAMUEL; UETANABARO, ANA PAULA TROVATTI; VINDEROLA, GABRIEL; NICOLI, JACQUES ROBERT
Revista:
ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY.
Editorial:
SPRINGER-VERLAG BERLIN
Referencias:
Año: 2017
ISSN:
0065-2598
Resumen:
In the present study, nine lactic acid bacteria isolated from the fermentationprocess of ?cupuac¸u? (Theobroma grandiflorum) were selected forprobiotic use. In vitro (resistance to gastrointestinal environment, in vitroantagonism and co-aggregation with pathogens) and in vivo (intestinalcolonization and ex vivo antagonism in germ-free mice, cumulative mortality,translocation to liver and spleen, histopathological examination ofliver and ileum and mRNA cytokine gene expression during an experimentalinfection with S. Typhimurium) assays were used. Among the nineLactobacillus strains isolated from the ?cupuac¸u? fermentation,L. plantarum 81 and L. plantarum 90 were selected as potential probioticsbased on better results obtained in in vitro evaluations (production ofdiffusible inhibitory compounds and co-aggregation) as well as in vivoexperiments (resistance to gastrointestinal environment, ex vivo antagonism,higher survival after enteropathogen challenge, lower hepatictranslocation of enteropathogen, lower histopathological lesions in ileum and liver and anti-inflammatory pattern of immunological response).Concluding, L. plantarum 81 and L. plantarum 90 showed in vitro andin vivo capacities for probiotic use through different mechanisms ofprotection and its origin would allow an easier adaptation in an alimentarymatrix for its administration.