INVESTIGADORES
VINDEROLA Celso Gabriel
artículos
Título:
Role of intestinal epithelial cells in the immune effects mediated by Gram (+) probiotic bacteria. Involvement of Toll-like receptors.
Autor/es:
C.G. VINDEROLA; C. MATAR; G. PERDIGÓN
Revista:
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY.
Editorial:
American Society for Microbiology
Referencias:
Lugar: Washington D.C.; Año: 2005 vol. 12 p. 1075 - 1084
ISSN:
1071-412X
Resumen:
The mechanisms by which probiotic bacteria exert their effects on the immune system are not completely
understood, but the epithelium may be a crucial player in the orchestration of the effects induced. In a previous
work, we observed that some orally administered strains of lactic acid bacteria (LAB) increased the number
of immunoglobulin A (IgA)-producing cells in the small intestine without a concomitant increase in the CD4
T-cell population, indicating that some LAB strains induce clonal expansion only of B cells triggered to
produce IgA. The present work aimed to study the cytokines induced by the interaction of probiotic LAB with
murine intestinal epithelial cells (IEC) in healthy animals. We focused our investigation mainly on the
secretion of interleukin 6 (IL-6) necessary for the clonal expansion of B cells previously observed with probiotic
bacteria. The role of Toll-like receptors (TLRs) in such interaction was also addressed. The cytokines released
by primary cultures of IEC in animals fed with Lactobacillus casei CRL 431 or Lactobacillus helveticus R389 were
determined. Cytokines were also determined in the supernatants of primary cultures of IEC of unfed animals
challenged with different concentrations of viable or nonviable lactobacilli and Escherichia coli, previously
blocked or not with anti-TLR2 and anti-TLR4. We concluded that the small intestine is the place where a major
distinction would occur between probiotic LAB and pathogens. This distinction comprises the type of cytokines
released and the magnitude of the response, cutting across the line that separates IL-6 necessary for B-cell
differentiation, which was the case with probiotic lactobacilli, from inflammatory levels of IL-6 for pathogens.Lactobacillus casei CRL 431 or Lactobacillus helveticus R389 were
determined. Cytokines were also determined in the supernatants of primary cultures of IEC of unfed animals
challenged with different concentrations of viable or nonviable lactobacilli and Escherichia coli, previously
blocked or not with anti-TLR2 and anti-TLR4. We concluded that the small intestine is the place where a major
distinction would occur between probiotic LAB and pathogens. This distinction comprises the type of cytokines
released and the magnitude of the response, cutting across the line that separates IL-6 necessary for B-cell
differentiation, which was the case with probiotic lactobacilli, from inflammatory levels of IL-6 for pathogens.Escherichia coli, previously
blocked or not with anti-TLR2 and anti-TLR4. We concluded that the small intestine is the place where a major
distinction would occur between probiotic LAB and pathogens. This distinction comprises the type of cytokines
released and the magnitude of the response, cutting across the line that separates IL-6 necessary for B-cell
differentiation, which was the case with probiotic lactobacilli, from inflammatory levels of IL-6 for pathogens.