VINCENT Paula Andrea
congresos y reuniones científicas
ENTEROBACTIN: A FENTON-SAFE SIDEROPHORE
PERALATA, DAIANA; ADLER, C.; CORBALÁN, NATALIA; PAZ-GARCÍA EC; POMARES, MARÍA FERNANDA; VINCENT, P.A.
Congreso; LII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2016
There is increasing evidence that siderophores may play alternative roles, aside of providing cells with the necessary iron. We previously reported that the catechol siderophore enterobactin, is crucial for Escherichia coli colony development in culture conditions that increased the oxidative stress. In this work, we demonstrated that enterobactin confers protection against various sources of oxidative stress such us H2O2, paraquat and copper, independently of its ability to facilitate iron uptake. Protection against oxidative stress occurs in the cell cytoplasm through ROS scavenging and requires prior hydrolysis of the enterobactin molecule. Interestingly, both the sensitivity to stressors and the colony development arrest phenotype were enhanced when cells harbored the entE mutation along with either sodA or katG. Confirming the link between enterobactin and oxidative stress, we found that enterobactin transcriptional expression and production was induced by oxidative stressors even in the presence of iron. Furthermore, preliminary data indicates that enterobactin transcription would be regulated by oxidative stress through the global regulator SoxS. These results strongly support the involvement of enterobactin as part of the oxidative stress response of E. coli.