MccJ25 C-terminal is involved in RNA-polymerase inhibition but not in respiration inhibition.

PAULA A. VINCENT, AUGUSTO BELLOMIO, BEATRIZ F. DE ARCURI, RICARDO N. FARÍAS, ROBERTO D. MORERO

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Elsevier

Año: 2005 vol. 331 p. 549 - 551

0006-291X

Microcin J25 appears to have two intracellular targets: (1) RNA polymerase, which was described in Escherichia coli and Salmonella enterica serovars, and (2) cell respiration in Salmonella enterica serovars. C-terminal glycine amidation of the threaded segment localized in the MccJ25 lariat ring region specifically blocked the RNA-polymerase inhibition, but not the cell respiration inhibition and peptide uptake. These results suggest that different regions of the molecule are responsible for each cellular effect, they are localized far away from the b-hairpin region and the C-terminal region is an important determinant for RNAP inhibition. localized in the MccJ25 lariat ring region specifically blocked the RNA-polymerase inhibition, but not the cell respiration inhibition and peptide uptake. These results suggest that different regions of the molecule are responsible for each cellular effect, they are localized far away from the b-hairpin region and the C-terminal region is an important determinant for RNAP inhibition. enterica serovars, and (2) cell respiration in Salmonella enterica serovars. C-terminal glycine amidation of the threaded segment localized in the MccJ25 lariat ring region specifically blocked the RNA-polymerase inhibition, but not the cell respiration inhibition and peptide uptake. These results suggest that different regions of the molecule are responsible for each cellular effect, they are localized far away from the b-hairpin region and the C-terminal region is an important determinant for RNAP inhibition. localized in the MccJ25 lariat ring region specifically blocked the RNA-polymerase inhibition, but not the cell respiration inhibition and peptide uptake. These results suggest that different regions of the molecule are responsible for each cellular effect, they are localized far away from the b-hairpin region and the C-terminal region is an important determinant for RNAP inhibition. Escherichia coli and Salmonella enterica serovars, and (2) cell respiration in Salmonella enterica serovars. C-terminal glycine amidation of the threaded segment localized in the MccJ25 lariat ring region specifically blocked the RNA-polymerase inhibition, but not the cell respiration inhibition and peptide uptake. These results suggest that different regions of the molecule are responsible for each cellular effect, they are localized far away from the b-hairpin region and the C-terminal region is an important determinant for RNAP inhibition. localized in the MccJ25 lariat ring region specifically blocked the RNA-polymerase inhibition, but not the cell respiration inhibition and peptide uptake. These results suggest that different regions of the molecule are responsible for each cellular effect, they are localized far away from the b-hairpin region and the C-terminal region is an important determinant for RNAP inhibition. serovars, and (2) cell respiration in Salmonella enterica serovars. C-terminal glycine amidation of the threaded segment localized in the MccJ25 lariat ring region specifically blocked the RNA-polymerase inhibition, but not the cell respiration inhibition and peptide uptake. These results suggest that different regions of the molecule are responsible for each cellular effect, they are localized far away from the b-hairpin region and the C-terminal region is an important determinant for RNAP inhibition.b-hairpin region and the C-terminal region is an important determinant for RNAP inhibition.
2005 Elsevier Inc. All rights reserved.2005 Elsevier Inc. All rights reserved.