INVESTIGADORES
VINCENT Paula Andrea
artículos
Título:
Canker Control by the Siderophore Pyochelin from Pseudomonas fluorescens
Autor/es:
ADLER, CONRADO; MICHAVILA, GABRIELA; FABIÁN LÓPEZ; CORBALÁN, NATALIA; LAMI, MARÍA JESÚS; FILIPPONE, M. PAULA ; CASTAGNARO, ATILIO; DE CRISTÓBAL, RICARDO; VINCENT, PAULA .A
Revista:
ACTA HORTICULTURAE
Editorial:
International Society for Horticultural Science
Referencias:
Año: 2015 vol. 1065 p. 937 - 945
ISSN:
0567-7572
Resumen:
This work shows the identification of a compound able to inhibit Xanthomonas citri subsp. citri (citrus canker agent) growth in vitro and in vivo. Firstly, we isolated from citrus leaves surfaces, bacteria able to inhibit X. citri subsp. citri in vitro. Among the selected isolates, we focused in one showing a remarkable activity. The strain was characterized as Pseudomonas fluorescens after sequencing its 16S rDNA and analyzing the sequence with BLASTn. Purification and chemical analysis of the active compound allowed us to assign the inhibitory activity to enantio-pyochelin (E-Pch). Since this molecule is a siderophore, we wondered if the inhibition observed was a result of iron scavenging. Surprisingly, when we supplemented media with an excess of iron, we observed practically no change in the inhibition activity. In an attempt to identify the action mechanism of E-Pch, we evaluated the ability of E-Pch to generate reactive oxygen species (ROS) within a culture of X. citri subsp. citri and its correlation with the inhibitory activity. In fact, we observed increased ROS levels when E-Pch was added. In addition, the reducer agent ascorbic acid, lowered ROS levels and the antibiotic activity, implying that inhibition is probably caused by oxidative stress. Finally, we studied the use of E-Pch in a model of canker disease. E-Pch showed to reduce canker formation on leaves of Eureka and Lisbon lemon cultivars. These results show E-Pch as a promising compound for citrus canker biocontrol.