INVESTIGADORES
VATTA Marcelo Sergio
artículos
Título:
Atrial natriuretic peptide reduces inflammation and enhances apoptosis in rat acute pancreatitis
Autor/es:
NAJENSON, A. C.; COURREGES, A. P.; PERAZZO, J. C.; RUBIO M; VATTA, M. S.; BIANCIOTTI LG
Revista:
ACTA PHYSIOLOGICA
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2018 vol. 222
ISSN:
1748-1708
Resumen:
AbstractAim: We previously reported that atrial natriuretic peptide (ANP) reduces serumamylase and intrapancreatic trypsinogen activation in the onset of acute pancreatitiswhereas secretin increases them. In the present work, we sought to establishthe effect of ANP and secretin on the inflammatory response and cell death inexperimental acute pancreatitis.Methods: The expression and activity of key inflammatory mediators and apoptosiswere evaluated in the presence or absence of the atrial peptide, secretin orboth in cerulein-induced acute pancreatitis in rats. Also, ultrastructural changes inpancreatic acinar cells were assessed by transmission electron microscopy.Results: ANP significantly reduced NF-jB activation and TNF-a intrapancreaticlevels. Furthermore, it decreased inducible nitric oxide synthase and cyclooxygenase 2expression and activity while it diminished myeloperoxidase activity. ANP also stimulatedapoptosis as shown by caspase-3 expression and activation as well as TUNELassay. These findings correlated well with the ultrastructural changes observed in theexocrine pancreas. Although secretin reduced various inflammatory markers, it alsodiminished caspase-3 activation and the overall response was the aggravation of thedisease as reflected by the ultrastructural alterations of pancreatic acinar cells. In thepresence of ANP, various effects evoked by secretin were antagonized.Conclusion: Present findings show that ANP significantly attenuated the severityof acute pancreatitis in the rat by inducing apoptosis and reducing the inflammatoryresponse and further suggest that ANP may have eventual therapeutic implicationsin the disease and/or in medical interventions at risk of its developing likeendoscopic retrograde cholangiopancreatography.