INVESTIGADORES
VATTA Marcelo Sergio
artículos
Título:
B and C Types Natriuretic Peptides Modify Norepinephrine Uptake and Release in the Rat Adrenal Medulla
Autor/es:
VATTA MS; PRESAS M; BIANCIOTTI LG; RODRIGUEZ FERMEPIN M; AMBROS R; FERNADEZ BE
Revista:
PEPTIDES
Editorial:
ELSEVIER SCIENCE INC
Referencias:
Lugar: Amsterdam; Año: 1997 vol. 18 p. 1483 - 1489
ISSN:
0196-9781
Resumen:
We have previously reported that atrial natriuretic factor (ANF) modulates adrenomedullar norepinephrine (NE) metabolism.On this basis, the aim of the present work was to study the effects of B and C types natriuretic peptides (BNP and CNP) on the uptake,intracellular distribution and release of 3H-NE. Experiments were carried out in rat adrenal medulla slices incubated ??in vitro.?? Resultsshowed that 100 nM of both, CNP and BNP, enhanced total and neuronal NE uptake. Both peptides (100 nM) caused a rapid increasein NE uptake during the first minute, which was sustained for 60 min. NE intracellular distribution was only modified by CNP (100nM), which increased the granular fraction and decreased the cytosolic pool. On the other hand, spontaneous as well as evoked (KCl)NE release, was decreased by BNP and CNP (50 and 100 nM for spontaneous release and 1, 10, 50 and 100 nM for evoked output).The present results suggest that BNP and CNP may regulate catecholamine secretion and modulate adrenomedullary biological actionsmediated by catecholamines, such as blood arterial pressure, smooth muscle tone, and metabolic activities.