Xenoestrogens exposure at environmentally relevant doses adversely affects ovine follicular development

RIVERA OE; VARAYOUD, JORGELINA; RODRIGUEZ HA; DI MAURO CE; MUÑOZ DE TORO, MÓNICA; LUQUE EH

Conferencia; 36th International Embryo Transfer Society, Annual conference; 2009

Follicular dynamics was established by histomorphological features on pricosirius-hematoxylin stained sections (at least 10 sections/ovary). The total number of follicles/ovary was obtained considering number of sections and section thickness (≥25,000 follicles/ovary). Follicles of different classes and multioocytes follicles were express as percentage.Immunohistochemical expression of estrogen receptor a (ERa), androgen receptor (AR), Ki-67 (proliferation marker) and p27 (cyclin-dependent kinase inhibitor 1B) was evaluated. Proliferative index was obtained using Ki67 expression in the granulosa (GC) and theca cells of antral follicles. Antral follicles with ≤1% Ki-67 were considered atretic. Kruskal-Wallis and Dunn post-test were used, p