New murine cell line derived from a spontaneous lung tumor induces paraneoplastic syndromes

ALEJANDRO J. URTREGER; MIRIAM J. DIAMENT; STELLA RANUNCOLO; SLOBODANKA M. KLEIN; MARÍA DEL CARMEN VIDAL; LYDIA I. PURICELLI; ELISA D. BAL DE KIER JOFFE

INTERNATIONAL JOURNAL OF ONCOLOGY

SPANDIDOS PUBL LTD

Año: 2001 vol. 18 p. 639 - 647

1019-6439

LP07 is a new cell line derived from P07 lung tumor, spontaneously arisen in a BALB/c mouse. LP07 is composed by heterogeneous epithelioid polyhedric cells that proliferate at a slow rate, have low plating efficiency and are unable to grow in soft agar. Only some LP07 cells expressed cytokeratins while most of them were positive for vimentin. Ultrastructure showed that LP07 cells established rudimentary intercellular unions, formed glandular-like conducts and presented conspicuous secretory granules, suggesting an epithelial-glandular origin, with neuroendocrine components. Upon injection LP07 cells formed poorly differentiated non-invasive adenocarcinomas, and tumor bearing mice developed leukocitosis, hipercalcemia and cachexia. This tumor cell line constitutes a useful tool to study lung tumor biology and paraneoplastic syndromes.