INVESTIGADORES
SORDELLI Daniel Oscar
artículos
Título:
Protein Antigens Increase the Protective Efficacy of a Capsule-Based Vaccine against Staphylococcus aureus in a rat model of osteomyelitis.
Autor/es:
LATTAR SM; NOTO LLANA M; DENOEL P; GERMAIN S; BUZZOLA FR; LEE JC; SORDELLI DO
Revista:
INFECTION AND IMMUNITY
Editorial:
AMER SOC MICROBIOLOGY
Referencias:
Lugar: Washington; Año: 2014 vol. 82 p. 83 - 91
ISSN:
0019-9567
Resumen:
Staphylococcus aureus is an invasive bacterial pathogen, and antibiotic
resistance has impeded adequate control of infections caused by this
microbe. Moreover, efforts to prevent human infections with
single-component S. aureus vaccines have failed. In this study, we
evaluated the protective efficacy in rats of vaccines containing both S.
aureus capsular polysaccharides (CPs) and proteins. The serotypes 5 CP
(CP5) and 8 CP (CP8) were conjugated to tetanus toxoid and administered
to rats alone or together with domain A of clumping factor A (ClfA) or
genetically detoxified alpha-toxin (dHla). The vaccines were delivered
according to a preventive or a therapeutic regimen, and their protective
efficacy was evaluated in a rat model of osteomyelitis. Addition of
dHla (but not ClfA) to the CP5 or CP8 vaccine induced reductions in
bacterial load and bone morphological changes compared with immunization
with either conjugate vaccine alone. Both the prophylactic and
therapeutic regimens were protective. Immunization with dHla together
with a pneumococcal conjugate vaccine used as a control did not reduce
staphylococcal osteomyelitis. The emergence of unencapsulated or
small-colony variants during infection was negligible and similar for
all of the vaccine groups. In conclusion, addition of dHla to a CP5 or
CP8 conjugate vaccine enhanced its efficacy against S. aureus
osteomyelitis, indicating that the inclusion of multiple antigens will
likely enhance the efficacy of vaccines against both chronic and acute
forms of staphylococcal disease.