INVESTIGADORES
SORDELLI Daniel Oscar
artículos
Título:
Fts insertional mutant of Salmonella typhimurium
Autor/es:
CERQUETTI MC; BRAWER R; GERDES CA; GHERARDI MM; SORDELLI DO
Revista:
FEMS MICROBIOLOGY LETTERS
Editorial:
Blackwell Publishers
Referencias:
Lugar: UK; Año: 1995 vol. 132 p. 73 - 78
ISSN:
0378-1097
Resumen:
A temperature-sensitive filamentation (fts) Salmonella typhimurium mutant was isolated after transposon mutagenesis with mini-Tn 10dTc. The mutant was unable to form colonies after 20 h incubation at 37 degrees C on LB agar. Colonies appeared, however, after longer incubation at the restrictive temperature. Filamentation affected only part of the bacterial population. Rapid mapping using Mu dP22 hybrid phages revealed that the mutation, ftsD220, lies within minutes 68.5 and 73.6 on the genetic map. Further analysis revealed that the ftsD220 mapped at min 73 and that it is linked to cysG (6%) and to aroB (39%). Complementation tests suggested that the ftsD220 mutation is not homologous to a Escherichia coli ftsH mutation