INVESTIGADORES
SORDELLI Daniel Oscar
artículos
Título:
Development of a plasmid vector for easy selection of strong promoters
Autor/es:
PIÑEIRO SA; SORDELLI DO; CENTRON D
Revista:
CURRENT MICROBIOLOGY
Editorial:
Springer International
Referencias:
Lugar: USA; Año: 2000 vol. 40 p. 302 - 305
ISSN:
0343-8651
Resumen:
A promoter vector pACPR33 for Escherichia coli based on the promotorless ampicillin-resistance gene from pBR322 has been constructed. The promoter of the ampicillin-resistance gene was deleted and replaced by a suitable multiple cloning site. Molecular cloning of promoters into the polylinker resulted in activation of the ampicillin resistance in E. coli. The plasmid contains a functional origin of DNA replication and a tetracycline resistance gene for E. coli, and a chloramphenicol resistance gene for S. aureus. The vector permitted direct detection of promoter activity, especially strong promoters, by easy iodometric determination of beta-lactamase activity in liquid or solid media
