INVESTIGADORES
SOMOZA Gustavo Manuel
congresos y reuniones científicas
Título:
Phylogenetic and gene/protein structure analysis of two paralogous kisspeptin receptors (kiss2r and kiss3r) in pejerrey (Odontesthes bonariensis).
Autor/es:
ALEJANDRO S. MECHALY; ARIEL E. MECHALY; OSVALDO TOVAR; JORDI VIÑAS; GUILLERMO ORTÍ; GUSTAVO M. SOMOZA
Lugar:
Bahía Blanca
Reunión:
Congreso; VI Argentinian Conference on Bioinformatics and Computational Biology.; 2015
Resumen:
Kisspeptin receptors play an essential role in the control of puberty in vertebrates. In this context, we report the gene organization, the in-silico structural analysis and phylogeny of two kissr-like receptors (termed Kiss2r and Kiss3r) in our experimental model, the pejerrey Odontesthes bonariensis.TBLASTN algorithm was used to retrieve the genomic sequences of Kiss2r and Kiss3r from the pejerrey genome database, to assess the genomic gene structure and the phylogenetic analysis using the coding sequences of the genes. Kiss2r and Kiss3r homology models were generated by using the GPCR modelling server GOMoDo. The total mRNA from different tissues was extracted with TRIZOL reagent and retrotranscribed, to then analyze the expression pattern by real-time quantitative PCR (qPCR).Phylogenetic analyses indicate that Kiss2r and Kiss3r paralog genes were probably originated by duplication that occurred early in teleost evolution. As in other species, the Kiss2r in pejerrey comprises five exons and four introns, and Kiss3r presents six exons and five introns. The tissue distribution expression profile by reverse transcriptase PCR evidenced two distinct transcripts for both receptors. Kiss2r contained an insert of 98 nucleotides caused by the retention of the whole intron III and Kiss3r an insertion of 79 bases, product of the whole intron IV retention. Furthermore, the predicted amino-acidic sequence for Kiss2r insert includes a premature termination codon in transmembrane helix 4. Kiss3r intron retention implies an open reading frame shift. Both splicing events probably result in non-functional and/or truncated receptors. The isoforms displayed distinct expression patterns in reproductive and non-reproductive related tissues.The non-truncated isoforms were observed mainly in different brain areas, pituitary and gonads, whereas the truncated isoforms were mostly detected in non-reproductive tissues.In conclusion, the differential expression pattern of the kissr isoforms may help to explain some discrepancies observed in past studies regarding pleiotropic expression of kissr. Though to assign a putative role (if any) for the truncated kissr isoforms would require further studies, it is tempting to speculate that the reported alternative splicing events might contribute to regulate kisspeptin/kissr mediated signalling pathways.