INVESTIGADORES
SOMOZA Gustavo Manuel
congresos y reuniones científicas
Título:
Characterization of secretoneurin variants in pejerrey (Odontesthes bonariensis).
Autor/es:
MICHAY DIEZ; OSWALDO TOVAR; GABRIELA C. LÓPEZ; VANCE L. TRUDEAU; GUSTAVO M. SOMOZA
Lugar:
Chascomus
Reunión:
Congreso; XVI Jornadas Anuales de la Sociedad Argentina de Biología; 2014
Institución organizadora:
Sociedad Argentina de Biología
Resumen:
Secretoneurin (SN) is a well conserved peptide from sharks to mammals, and it is the product of the cleavage of the larger precursor Secretogranin II (also called Chromogranin C). Nevertheless a receptor for SN has not been found yet; SN has been proposed to be a new hormone. Also, in some teleost fish species two SN variants have been reported and called as SNa and SNb. In this context we attempted to characterize SN variants in pejerrey and their neuroanatomical distribution to then start looking for their function. Consensus degenerated primers were designed after alignment of SNa and SNb forms in other teleost species and used on cDNAs obtained from pejerrey brains and pituitaries. Partial SNa and SNb sequences were obtained in both tissue extracts. Also pejerrey brains were treated for immunohistochemistry using a SN antiserum raised against goldfish SNa. SN-immunoreactive (SN-ir) cells were observed in pejerrey magnocellular and parvocellular cells of the preoptic nucleus with their projections ending at the neural lobe of the pituitary gland. Also, some SN-ir cells were observed in the pituitary gland, however up to the moment the nature of these cells is under study. In conclusion we report for the first time the presence of SN variants in pejerrey brain and pituitary to start studies on their physiological functions.