INVESTIGADORES
SOMOZA Gustavo Manuel
congresos y reuniones científicas
Título:
Cloning, characterization and tissue distribution expression pattern of two GnRH receptors in pejerrey. Phylogenetic considerations.
Autor/es:
LEONARDO GASTÓN GUILGUR; GUSTAVO M SOMOZA
Lugar:
Saint Malo. Francia
Reunión:
Congreso; 8th International Symposium on Reproductive Physiology of Fish.; 2007
Resumen:
BACKGROUND: The events leading to GnRH-stimulated gonadotropin synthesis and release from the anterior pituitary gland is dependent on the presence of gonadotropin releasing hormone receptors (GnRHRs). Today it is well known that multiple GnRHRs are expressed in a single species. In this context, the main objective of the present study was to characterize the cDNA sequences encoding GnRHR in our model species, the pejerrey Odontesthes bonariensis, document their expression pattern, and also present a phylogenetic analysis including representatives of other chordate GnRHR. METHODS: Complementary deoxyribonucleic acid from brain/pituitary tissue of adult pejerrey, was used to perform RACE PCR. Then, RT-PCR analysis was performed with specific primers in different brain areas and extra-nervous tissues and organs in order to examine the distribution of GnRHR mRNAs. Phylogenetic maximum parsimony trees were generated with the TNT program and Jackknife re-sampling with 1,000 replicates was performed. RESULTS: Two full-length GnRH receptors were cloned, sequenced and their structure characterized. The expression pattern profile showed that both receptors were widely expressed both in the central nervous system and also in peripheral organs. Differences in the tissue distribution expression of both receptors were determined. The phylogenetic analysis resulted into two main lineages of GnRHRs. CONCLUSION: The two GnRHRs molecules obtained share the structural organization of the other vertebrate GnRHRs sequences. The expression data analysis suggests that in pejerrey GnRHRs could be mediated directly or indirectly many functions besides reproduction and the existence of GnRH systems independent of hypothalamic–pituitary–gonadal axis. The phylogenetic results allowed hypothesizing that these molecules can be grouped into two main types as already proposed.