INVESTIGADORES
SOMOZA Gustavo Manuel
congresos y reuniones científicas
Título:
The gonadotrophin-releasing hormone receptors: a factor in fish osmoregulation?
Autor/es:
ADELINO CANARIO,; P. GUERREIRO; N SILVA; DEBORAH POWERS; GUSTAVO M SOMOZA; NATALIA MONCAUT
Lugar:
Manchester, UK
Reunión:
Congreso; 23rd Conference of European Comparative Endocrinologists; 2006
Institución organizadora:
European Society of Comparative Endocrinology
Resumen:
The gonadotrophin-releasing hormone receptors: a factor in fish osmoregulaton? Canário, A.V.M.,  Guerreiro, P.M., Silva, N., Power, D.M., Somoza, G.§ and Moncaut, N.   Centre of Marine Sciences, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal; § Laboratorio de Ictiofisiologia y Acuicultura, Instituto de Investigaciones Biotecnologicas-Instituto Tecnologico de Chascomus, Camino de Circunvalacion Laguna Km. 6 (B7130IWA). Chascomús, Provincia de Buenos Aires, Argentina. E-mail: acanario@ualg.pt     In a previous study we have shown that the genome of European sea bass Dicentrarchus labrax, and possibly of most teleosts, contains five functional gonadotrophin-releasing hormone receptors which belong to two main families GnRHR-1 and GnRHR-2. Based on the analysis of gene expression in multiple tissues we have hypothesized that in addition to the known roles of the GnRH system in reproduction, one or several of the receptors could be involved in osmolyte regulation. The present study was designed to analyse the mRNA expression levels of GnRHR-1A and -1B in brain, pituitary, olfactory epithelium, gills and kidney in European sea bass by real time reverse transcription-polymerase chain reaction and in situ hybridisation after transfer from seawater (SW) to freshwater (FW). Transfer to FW induced significant alterations on transcript levels of both receptors, to a different extent, in all tissues sampled. Receptors were localized in distinct cell types and regions in the tissues analysed. For example, GnRH-1B was detected in cells of the pars intermedia of the pituitary gland and in Rodlet cells of the olfactory epithelium. The data strongly suggests that GnRHR-1 signalling is likely to be involved in the mechanisms of fish osmoregulation.