INVESTIGADORES
SOMOZA Gustavo Manuel
artículos
Título:
Expression profile and estrogenic regulation of anti-Müllerian hormone during gonadal development in pejerrey Odontesthes bonariensis, a teleost fish with strong temperature-dependent sex determination.
Autor/es:
JUAN IGNACIO FERNANDINO; RICARDO HATTORI; HIROYUKI KIMURA; CARLOS AUGUSTO STRÜSSMANN; GUSTAVO M. SOMOZA
Revista:
Develpmental Dynamics
Editorial:
Wiley
Referencias:
Año: 2008 vol. 237 p. 3192 - 3199
ISSN:
1058-8388
Resumen:
Pejerrey is a teleost fish presenting a strong temperature-dependent sex determination.  This study was conducted to clone pejerrey amh cDNA, analyzed its expression profile during thermal and endocrine manipulation of gonadal differentiation, and compare its expression to that of gonadal aromatase (cyp19a1).  Amh displayed higher expression at masculinizing than at feminizing temperatures during the gonadal differentiation period.  Its expression at an intermediate temperature (females 1:1 males), was high in half of the larvae and low in the other half.  Cyp19a1 showed a reciprocal expression pattern to that of amh both individually- and temperature-wise.  Increased cyp19a1 and amh expression was observed before morphological gonadal differentiation.  Amh expression in larvae feminized by administration of estradiol or masculinized by the administration of an aromatase inhibitor was down- and up-regulated, respectively.  These results show that amh plays a critical role in testicular differentiation and it is apparently modulated by estrogens in this species.