SEIJO Jose Guillermo
congresos y reuniones científicas
Informative chloroplast regions in Ilex paraguariensis and Ilex affinis, for phylogenetic and species identification
STEPHANIE TALAVERA LILIANA; FAY J V; PERCUOCO C B; ROJAS C; MIRETTI M; SEIJO J G; ARGUELLES C
Congreso; International Plant Molecular Biology Congress; 2015
Argentina contains seven species of the genus Ilex L. (Aquifoliaceae), including I. paraguariensis, which is economically important, and I. affinis, a shrubby species of riparian habitat that was recently cited as occurring in Atlantic Forest. Knowing that within the genus the phylogenetic relationships of I. affinis are still unknown and there is a need for makers that can be used to determine dopants in I. paraguariensis leaves products (yerba mate), this work aimed to characterize different chloroplast regions that could help in establishing phylogenetic relationships between I. affinis and other species of the genus in South America, as well as to differentiate the species. A total of 15 individuals (I. paraguariensis= 11, I. affinis= 4) were collected from five distant natural and cultivated populations of I. paraguariensis from Argentina and Paraguay and a natural population of I. affinnis from Argentine. The analyses of three chloroplast regions, one intron [trnL] and two intergenic spacers [petG-trnP, psbJ-petA] identified one haplotype for each species showing no intraspecific differences. However, interspecific haplotype/nucleotide differences were observed (transitions, transversions and InDels). The alignment of 469 bp of the trnL intron was used in the phylogenetic relationships analysis including sequences of eight species of the genus available in GenBank and considering Helwinia japonica as outgroup. Two clusters were defined: one grouping Brazilian species and the second grouping Argentine species. Inside the latter, I. affinis and I. dumosa were grouped (BS: 0.89). Also, the data suggested that the three tested regions might be useful in species identification.