SEIJO Jose Guillermo
congresos y reuniones científicas
Genomic characterization of the germplasm in peppers (Capsicum Solanaceae) by fluorescent in situ hybridization.
SCALDAFERRO, M.A; J.G. SEIJO; M. C. ACOSTA; G. E. BARBOZA; D. A. DUCASSE; E A. MOSCONE.
Simposio; Bulgarian Symposium on Agriculture; 2005
National Centre for Agrarian Sciences
Genomic characterization of the germplasm in peppers (Capsicum Solanaceae) by fluorescent in situ hybridization Marisel A. Scaldaferro1, J. Guillermo Seijo1, M. Cristina Acosta1, Daniel A. Ducasse2 and Eduardo A. Moscone1 1 Multidisciplinary Institute of Plant Biology (IMBIV), National University of Córdoba-CONICET, Casilla de Correo 495, 5000 Córdoba, Argentina. firstname.lastname@example.org 2 Institute of Phytophatology and Plant Physiology (IFFIVE), INTA, Camino a 60 Cuadras Km 5½, 5119 Córdoba, Argentina. Capsicum, an economically important New World genus of food, spice, drug and ornamental uses, includes worldwide crops (sweet and hot peppers). In this work we have started a broad genomic characterization programme of peppers germplasm by fluorescent in situ hybridization (FISH), with the aim of contributing to the knowledge of the genome organization and evolution in Capsicum, with reference to the origin of the crop species. It is expected to achieve useful information for breeding purpose concerning the cytogenetic diversity of the germplasm and the genomic affinity between the possible donors of valuable alleles and the crops to be improved. Two wild (C. chacoense, C. cardenasii) and three cultivated taxa (C. annuum var. annuum two cultivars , C. baccatum var. umbilicatum, C. pubescens) are analysed. The combination of the 45S and 5S rRNA gene probes in single FISH experiments brings useful chromosome markers to distinguish cytogenetically species, varieties and cultivars. Our results are discussed with previous findings in the genus. This work was supported by the International Atomic Energy Agency (United Nations Organization, Vienna), grant no. RBF 12226.