INVESTIGADORES
SEIJO Jose Guillermo
congresos y reuniones científicas
Título:
Expression analysis of N19, a gene related with sexual and apomictic development in Paspalum notatum
Autor/es:
SARTOR, MA; ESPINOZA F; SEIJO JG; GONZALEZ, AM; S.C. PESSINO
Reunión:
Congreso; XV Congreso Latinoamericano de Genética; 2012
Resumen:
EXPRESSION
ANALYSIS OF N19, A GENE
RELATED
WITH SEXUAL AND APOMICTIC
DEVELOPMENT
IN Paspalum notatum
Sartor ME1,
F Espinoza1, G Seijo1, AM González1,
S Pessino2.
1Instituto de Botánica del Nordeste
(IBONE-CONICET),
Facultad de Ciencias
Agrarias, UNNE, 2Consejo Nacional de
Investigaciones Científicas
y Técnicas (CONICET), Facultad de
Ciencias Agrarias,
Universidad Nacional de Rosario.
e-mail:
mariasartor@agr.unne.edu.ar
In
former expression analysis aimed at identifying
genes
differentially expressed in reproductive
tissues
of apomictic and sexual genotypes, we
isolated
a differentially expressed tag (experimental
code
N19), which kept similarity with checkpoint
homologue
CHK1. This candidate gene showed a
high
expression level, and was found upregulated
in
sexual plants. The objective of this work was to
better
characterize N19 structure and expression in
reproductive
tissues of apomictic and sexual plants
of P. notatum. Relative expression
quantitation
using
q-RTPCR revealed heterochronic temporal
expression
patterns, with minor upregulation
in
apomictic plant at premeiosis and meiosis,
downregulation
at postmeiosis and equal expression
at
anthesis. Spatial expression patterns were
examined
by using in situ RNA hybridization on
reproductive
tissues at meiosis and anthesis. During
meiosis,
the antisense probe revealed a strong signal
in
the teguments and nucella of the apomictic plant,
but
only in the area of the megaspore mother cell
of
the sexual plant. In anthesis, both reproductive
types
(apomictic and sexual) displayed a uniform
expression
pattern when hybridized with the
antisense
probe, displaying signal in teguments, egg
apparatus
and polar nuclei. No signal was observed
with
the sense probe. Rapid amplification of cDNA
ends
(RACE) experiments allowed the isolation of
single
bands, which will be sequenced in order to
characterize
the transcript full-length structure. Our
results
showed that the expression pattern of N19
actually
differs between both reproductive types.
Journal
of Basic & Applied Genetics. Suppl. Vol XXIII (1) 2012
GGM
165