AHCYL1 as a potential player in lung cancer

BUDNIK, NICOLÁS; CASTRO, ARACELI; MUÑOZ BERNART, MELINA; THOMAS, LAURA; PEREZ CASTRO, CAROLINA; CANEDO, LUCIA; VIGLIANO, CARLOS

Capital federal

Congreso; Reunión Anual de Sociedades de Biociencias 2020; 2020

Sociedad Argentina de Investigación Clínica

AHCYL1 is a protein involved in cellular homeostasis. However, its potentialcontribution to pathogenesis, in particular cancer, remains unclear. We aim toexplore its role focusing on lung cancer. AHCYL1 KD by shRNA caused anupregulation of pluripotency markers (such as POU5F1, CD133, and CD44), inaddition to higher sphere-formation capacity in vitro and tumorigenesis in vivo. Ameta-analysis of transcriptomic data from public databases (TCGA) revealedAHCYL1 is expressed in all grade II-III lung cancer samples, but is inverselycorrelated to stemness-related genes and directly correlated to differentiationgenes expressions. Moreover, AHCYL1 is upregulated primary biopsies of lungcancer compared to relapsed tumor samples characterized by higher tumorigenicpotential. Preliminary data of immunohistochemical analysis in lung cancer patientsamples revealed less AHCYL1 positive staining in samples corresponding tohigher stages. Statistical significance was evaluated considering acut-off p-value ≤ 0.05. Understanding AHCYL1?s role in lung cancer wouldcontribute to better strategies for diagnosis and therapy.