Estudio del gen Ahcyl1 en un modelo de plasticidad de células tumorales.

NICOLAS BUDNIK; MELINA MUÑOZ BERNART; CAROLINA PEREZ CASTRO

Congreso; Reunión Conjunta de la Sociedad de Investigación Clínica; 2018

Development and tumorigenesis involve cell plasticity events, where cells mayundergo reprogramming. TGFβ/SMAD proteins modulate the behavior of stemcells as well as the tumor cells. The transcriptional factors OCT4, SOX2 andNANOG constitute a ?core? factor essential for the self-renewal and pluripotencyproperties of stem cells and are expressed in tumor cells with stem cell properties.Our aim is to study cell plasticity events in a tumor model. In this project we usedA549 human lung adenocarcinoma cell line, because is capable of growingdeprived of serum in low attach plates and is induced to undergo TGF-beta-driven EMT. We have co-developed INSECT, a bioinformatic tool to identify genespotentially co-regulated by the Core factors and SMADs in stem cells. Using thistool we identified and started to characterize Ahcyl1 gene. Results showed thatafter 48-hour-TGFbeta1 treatment Ahcyl1 transcriptional variant A isdownregulated (p