Phosphorus deficiency in soybean plants

GALATRO A; RAMOS-ARTUSO F; LIMA; BATTHYÁNY DIGHIERO; SANTA MARIA G.E.; SIMONTACCHI M

Mar del Plata

Congreso; LI Reunión Anual Sociedad Argentina de Investigaciones Bioquímicas; 2015

SAIB

PHOSPHORUS DEFICIENCY IN SOYBEAN PLANTSGalatro A1, Ramos Artuso F2, Lima A3, Batthyány Dighiero C3, Santa María GE4,Simontacchi M2. 1 IBIMOL UBA-CONICET, 2 INFIVE UNLP-CONICET, 3 Institut Pasteur de Montevideo, 4 INTECH UNSAM-CONICET. E-mail: agalatro@ffyb.uba.arPhosphorus (P) is a macronutrient necessary for energy storage and anintegral component of several compounds in plant cells. Limitation of cropproductivity by P is widespread and is likely to increase in the coming years.Plants have evolved different mechanisms to cope with low soil P-availabilitysuch as modification of root architecture, and exudation of organic acids.Nitric oxide (NO) plays important roles in plants, being involved inphysiological processes and stress responses. The hypothesis of this work wasthat NO participates in early soybean responses to P restriction. Seven-day-oldplants were exposed for 24 h to a medium lacking P. Total P concentrationdecreased significantly in roots (6.6 ± 0.6 and 3.5 ± 0.8 μmol/g FW in P+ andP-, respectively) and leaves (10 ± 1 and 5.8 ± 0.2 μmol/g FW in P+ and P-, respectively).Electron paramagnetic resonance (EPR) and fluorescence microscopy were employedto assess endogenous NO levels. Confocal laser microscopy (employing DAF-FMDA)clearly showed higher NO level in P- leaves. Analysis of nitrotyrosines bytwo-dimensional PAGE/western blot/Mass spectrometry showed an increase inprotein tyrosine nitration in leaves of P- plants. In addition, differentialleaf proteins expression was analyzed by nLC-ion trap MS. These results suggestthat NO is involved in the early events related to P scarcity. PICT 2012-0429