INVESTIGADORES
RUIZ Oscar Adolfo
congresos y reuniones científicas
Título:
Isolation and characterization of Lotus spp. copper transporters
Autor/es:
PEREA GARCÍA A.; ESCARAY F.J.; ANTONELLI C.J.; COPELLO G.; PEÑARRUBIA L.; PUIG S.; RUIZ O.A.
Lugar:
CORDOBA
Reunión:
Congreso; XXXII REUNIÓN ARGENTINA DE FISIOLOGÍA VEGETAL - XVI CONGRESO LATINOAMERICANO DE FISIOLOGÍA VEGETAL; 2018
Institución organizadora:
SAIB
Resumen:
Forage legumes are important resource for livestock; particularly, Lotus spp. developed a key role into cattle agrosystems at Flooding Pampas (FP) region (Argentine). Occurrence of hypocuprosis in calves are often observed in FP due in part by low copper content in forage produced by natural grassland. Our previous results showed that pastures including Lotus spp. offer better copper levels in forage than those produced by natural pastures. However, little is known about the biological process and molecular mechanisms involved into copper homeostasis displayed by Lotus spp. Here we identify and isolate four genes that codified proteins related to high affinity copper transporter (CTR). Complementation assay with Saccharomyces cerevisiae ctr1ctr3Δ strain of the Lotus CTRs proteins showed a partially restoration of yeast growth in selective media. Moreover, the relative expression of CTRs genes and copper contents from L. japonicus plants was evaluated under control and waterlogging conditions. Upon this condition the copper uptake decrease and also induce the gene expression of some of these CTRs. Obtained results suggest that the four identified genes codify for active CTR proteins. Finally, environmental conditions affects the expression of two genes, suggesting an adaptive mechanisms to copper availability.