INVESTIGADORES
RUIZ Oscar Adolfo
congresos y reuniones científicas
Título:
Physiological keys to elucidate the differential response of Lotus spp.- Fusarium solani interaction
Autor/es:
NIEVA, A.S.; ERBAN A.; FEHRLE I.; KOPKA J.; RUIZ O-A.
Lugar:
Foz do iguazu
Reunión:
Simposio; Symposium of Biological Nitrogen Fixation in Non Legumes-RELARE 2018; 2018
Institución organizadora:
EMBRAPA
Resumen:
The interactions between plants and fungal endophytes could improve the host performance. Nevertheless some agents, such as the environment, plant species and age could modify the development of the system, changing the balance from mutualism to pathogenesis. This variability among fungal effect on plants has led the mutualism-parasitism continuum theory. Lotus tenuis, is a widely legume used as forage in Argentina, due of its capacity of growth in restrict soil conditions. Additionally, the model Lotus japonicus has been used for the study of responses to biotic and abiotic stresses. Our aim was focused in to determinate the main factors involved in the response of two Lotus species (L. japonicus and L. tenuis) to the symptomless infection of Fusarium solani. This strain has been capable to infect roots of L. tenuis and L. japonicus. Both infections were intra-cellular, restricted to the root surface, epidermis and cortex. The characterization of the physiological response of both plant species to the effect of F. solani, was performed through physiological analysis of photosynthesis and chlorophyll fluorescence parameters. In addition, through the analysis of metabolic profile, using GC-EI/TOF-MS technology, we were determinate the main compounds involved in the primary metabolism. Our results have shown a growth improvement in plants of L. japonicus, displaying as the increase of biomass of shoots and roots; while in L.tenuis, there was a decrease in the same parameters. Photosynthetic rates did not to be affected by the infection, in both species. The metabolic profiling has shown the differences in the amount of some sugars, phosphates, phenylpropanoids and polyols. Taking together, our results indicate a differential response of Lotus species to the infection by a Fusarium endophyte. This evidence could elucidate the base of the study of some mechanisms that could explain the switch between mutualism and pathogenesis, for this proposed biological model.