INVESTIGADORES
RUIZ Oscar Adolfo
congresos y reuniones científicas
Título:
IRON ACQUISITION IN LOTUS CROPS SUBJECTED TO ALKALINE STRESS: RESPONSE TO INOCULATION WITH Pantoea eucalypti M91.
Autor/es:
CAMPESTRE M.P; CASTAGNO N; ANTONELLI C.J.; MAGUIRE V.; PEREA GARCIA, A; ESCARAY F.J.; RUIZ, OSCAR A.
Lugar:
BUENOS AIRES
Reunión:
Congreso; REUNION CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017
Institución organizadora:
SOCIEDADES DE BIOCIENCIAS (AD HOC)
Resumen:
Alkalinesoils are characterized by low availability of essential nutrients, includingiron (Fe), causing Fe chlorosis and therefore affecting plant growth. Inaddition, soil microorganisms, through production of siderophores, may play animportant role capturing this element under limited conditions. Here, we aimed to study the response of L. tenuis (Lt) and L.corniculatus (Lc) and an interspecific hybrid LtxLc obtained in ourlaboratory to alkaline treatment (10mM NaHCO3) and inoculation with P. eucalypti M91 (PM91), a plantgrowth-promoting bacterial strain capable of producing siderophores at high pH.At first, we observed that alkalinity affected shoot growth of Lc (p=0.0049)and developed chlorosis in their leaves. Interestingly, when inoculated with PM91,Lc exhibited an increase in shoot growth (p=0.039). On the other hand, no detrimentaleffect was seen in growth of shoots and roots of Lt and LtxLc when exposed toalkalinity, but no effect of inoculation was observed either. Moreover, the Fe2+content in shoots of Lc and LtxLc when cultivated under alkaline conditions waslower than controls (p≤0.01), but when inoculated, the Fe2+ contentin shoots increased in all cases obtaining even greater values than the onesreached at controls. In addition, all species increased their Fe2+ rootcontents under alkalinity, compared to controls. Furthermore, the Fe2+accumulation was higher in the roots of alkaline treated Lc (2-fold) than inthe other species. These results, together with the chlorosis observed in Lcgrown under alkalinity, reveal a flaw in Fe2+ translocation from rootsto shoots. However, inoculation improved the Fe2+ balance for thisspecies, resulting in high endogenous Fe2+ content in the shoots,with accompanying re-greening in young leaves. These findings suggest thatparticularly, Lc is the species more affected to alkaline stress; howeverinoculation with PM91 generates an increase in the Fe2+ content ofthe three studied accessions. Keywords: Alkalinity,Lotus spp., Fe, endophytic bacteria.