POLYAMINE CONCENTRATIONS ARE DYNAMICALLY REGULATED DURING PLANT-PSEUDOMONAS SYRINGAE INTERACTIONS

ROSSI F.R.; VILAS J.M.; ROMERO M.; RUIZ, OSCAR A.; GARRIZ, A.

BUENOS AIRES

Congreso; REUNION CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017

SOCIEDADES DE BIOCIENCIAS (AD HOC)

Due to theirabilities to bind polyanions such as nucleic acids and proteins, the polyaminesputrescine, spermidine and spermine are involved in several cellular responses.For instance, these amines participate in the activation of plant defenseresponses and are required for virulence of plant pathogens. Thus, themodulation of their metabolism plays essential roles in plant-pathogeninteractions. In this work, we challenged plants with different pathovars ofthe bacteria Pseudomonas syringae toevaluate the changes in the concentrations of polyamines occurring in contrastingpathogenic interactions. Our analysis demonstrated that polyamines areincreased in tomato in response to a virulent strain, which was associated to theinduction of several polyamine metabolic genes from plant and bacteria. Theseresults suggest that the metabolism of both organisms modulates polyamine contentsin infected tissues. Interestingly, the rise in the concentration of putrescinewas particularly higher in apoplastic fluids. A further exploration of the polyamineprofiles using Arabidopsis plantsdemonstrated that non-pathogenic strains inducing the hypersensitive responseprovoked a remarkable increment in their concentrations, which was not observedby using an avirulent non-host bacteria. Thus, specific regulatory mechanisms maymodulate polyamine metabolism in different biological interactions.