INVESTIGADORES
RUIZ Oscar Adolfo
congresos y reuniones científicas
Título:
THE PGPR BURKHOLDERIA SP. (AU4i) PROTECTS ARABIDOPSIS THALIANA AGAINST FUNGAL PATHOGENS THROUGH DIRECT INHIBITION AND MODULATION OF DEFENSE AND PLANT CELL WALL METABOLISM
Autor/es:
COLAVOLPE B.; GARRIZ A.; VILLARREAL N.; ROSSI F.R.; ROMERO F.M.; RUIZ O.A.; SAINI A.; MARINA M.
Lugar:
BARCELONA
Reunión:
Congreso; CONGRESO DE LA SOCIEDAD DE FISIOLOGÍA VEGETAL ESPAÑOLA; 2017
Institución organizadora:
SOCIEDAD ESPAÑOLA DE FISIOLOGIA VEGETAL
Resumen:
Burkholderia sp. (AU4i), a strain which can acts as PGPR (Plant Growth-Promoting Rhizobacteria) in plants, was previously isolated from pea. We demonstrated that AU4i is able to grow endophytically on Arabidopsis plants and promote Arabidopsis growth increasing the number of lateral roots as well as root and shoot dry weight. To evaluate the potential of AU4i as a biocontrol agent, leaves inoculated with AU4i were challenged with the necrotrophic pathogens Botrytis cinerea and Sclerotinia sclerotiorum. The necrotic lesion areas provoked by both pathogens were reduced in leaves previously inoculated by AU4i. Additionally, AU4i was capable to inhibit the in vitro growth of B. cinerea, but not of S. sclerotiorum. In other hand, AU4i was able to induce the expression of the jasmonate responsive marker gene PDF1.2, but not the expression of salicylic acid marker gene (PR1). The protective effect of AU4i against fungal pathogens was evaluated in Arabidopsis defense signaling pathways mutant plants, coi1 (defective in jasmonate perception) and sid2-2 (defective in salicylic acid synthesis). Similar protective effect was observed in both lines in comparison to Col0 plants, suggesting the independence of the AU4i protective effect and plant signaling pathways. As well, the effect of AU4i inoculation on plant cell wall metabolism during that endophytic interaction was evaluated. As result it was revealed that AU4i modulates host cell wall metabolism, through the down-regulation of genes involved in hemicelluloses and pectin lateral chains degradation (AtExp8 and AtAra1, respectively) and the up-regulation of AtPME3, a gene encoding a putative pectin methyl esterase involved in pectin main chain esterification. Taken together, our results suggest that Burkholderia sp. AU4i might be a good candidate as a biocontrol agent.