INVESTIGADORES
RUIZ Oscar Adolfo
capítulos de libros
Título:
Phenotypic and Genotypic Characterization of Mutant Plants in Polyamine Metabolism Genes during Pathogenic Interactions
Autor/es:
ROSSI F.R.; ROMERO F.M.; RUIZ O.A.; MARINA M.; GARRIZ A.
Libro:
PLANT POLYAMINES: METHODS AND PROTOCOLS
Editorial:
SPRINGER
Referencias:
Lugar: NEW YORK; Año: 2018; p. 405 - 416
Resumen:
Plants respond to pathogen attack by modifying defense gene expression and the production of myriad proteins and metabolites. Among these responses, polyamine (PA) levels suffer remarkable modifications. Evidences demonstrate that plants make use of the polyamine biosynthetic pathway and the oxidative catabolism of these compounds in order to mount adequate defenses against pathogens. In Arabidopsis thaliana, putrescine is synthesized exclusively by the arginine decarboxylase (ADC) enzyme, which exists as two isoforms named ADC1 and ADC2. Even though both isoforms were described to participate in the response to pathogen attack, the regulatory mechanisms modulating ADC activity are not completely understood. Therefore, studies to clarify their roles are necessary. In this chapter, we describe the methods that can be applied for the study of plant-pathogen interactions using Arabidopsis ADC mutant plants.