IBAM   22618
INSTITUTO DE BIOLOGIA AGRICOLA DE MENDOZA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
lncRNAs involvement in stress-related plasticity in Sugar beet
Autor/es:
CHIALVA, CONSTANZA; LIJAVETZKY, DIEGO
Lugar:
Bernal, Buenos Aires
Reunión:
Otro; II Reunión Argentina de Biología de ARNs no codificantes; 2018
Institución organizadora:
Universidad Nacional de Quilmes
Resumen:
Sugar beet (Beta vulgaris L.) crop is the second more important source of sucrose after sugarcane. Currently, INTA is conducting research to study the adaptation of this crop in the Buenos Aires Valley of the Colorado River for the subsequent production of alcohols for biofuels. In Mendoza, only a few hectares of beet cultivated for horticultural consumption are registered. Given that the genetic diversity of the cultivated sugar beet varieties is low, the recent knowledge of the reference genome will allow establishing the genetic basis to promote the improvement of beet favoring a high and stable productivity of sugars. This productivity is highly affected by conditions of salt and water stress, and by the low temperatures outside the crop season. These cultural problems that are present in the Cuyo region, support the development of a study with these characteristics in order to establish this crop in the region.At present it has been shown that RNA, like DNA, can give orders, exceeding the role of messenger. Additional RNAs that do not code for proteins, such as long non-coding RNAs (lncRNAs), can carry out the reprogramming of gene expression at both the transcriptional, post-transcriptional and post-translational levels. This source of plasticity allows plants with the same genotype to show different phenotypes in response to varied stress conditions.In order to establish the genetic and epigenetic bases that explain the regulation of plasticity of some varieties of sugar beet and to define those that best adapt to the growing conditions in our region, we propose to carry out a global transcriptomic analysis in the root tissues of a variety with high productive yield (cv. Magnolia) under saline and hydric stress, or exposed to low temperatures. This study seeks to identify and characterize a number of lncRNAs that respond to the stress condition to which the culture is subjected, in order to determine the target genes on which they act, and finally to evaluate their co-expression (lncRNA-target) in other high productivity genotypes (cvs. Antonella and Victoriana). Likewise, the epigenetic regulation carried out over lncRNAs and thus their role in the activation of target genes will be studied.