IBAM   22618
INSTITUTO DE BIOLOGIA AGRICOLA DE MENDOZA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
DISPERSIVE SOLID-PHASE EXTRACTION AND IN-VIAL FILTRATION AS A SIMPLIFIED CLEAN-UP FOR ETHYLPHENOLS DETERMINATION IN RED WINES
Autor/es:
BOTTINI, R.; FONTANA, A.
Lugar:
Chicago
Reunión:
Conferencia; 2017 Pittsburg Conference on Analytical Chemistry & Applied Spectroscopy (Pittcon); 2017
Institución organizadora:
Pittsburgh Conference on Analytical Chemistry and Applied Spectroscopy
Resumen:
[Brettanomyces] sp. is a microorganism related to several wine faults, most notably those known as phenolic off-flavor or Brett character. This flavor can be described as horsey, leathery, medicinal, smoky or savory being caused by the presence of ethylphenols (EPs). Although Brettanomyces is considered a spoilage organism that cause an objectionable flavor in red wine when their related spoilage compounds are present in high levels, low levels of EPs are sometimes considered adding complexity to a wine. Due to the relevant organoleptic impact of EPs on wine quality at variable levels, the development of analytical methodologies for the determination of them in wine samples is a subject of interest. In this work, we report the development and validation of a new high-throughput analytical method to monitor EPs in red wines using a quick, easy, cheap, effective, rugged and safe (QuEChERS) sample preparation followed by HPLC-UV determination. The approach combines in-vial filtration with dispersive solid-phase extraction (d-SPE) in a fast and convenient clean-up of QuEChERS extracts. During method development, different commercial sorbents for d-SPE were investigated and compared with respect to analyte recoveries. The method was validated at 0.1, 0.5 and 5 mg L-1 spiking levels, and the results for all analytes recoveries were between 72 and 119 % with RSD ≤ 15 %. The developed method was applied to several samples; being 4-ethylphenol and 4-vinylguaiacol the compounds detected with major frequency. The presented approach has high sample throughput and usefulness in screening studies for wines Brett character analysis.