IBAM   22618
INSTITUTO DE BIOLOGIA AGRICOLA DE MENDOZA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Isolation, morphological and molecular characterization of entomopathogenic fungi as a potential control strategy against Lobesia botrana in Argentinean vine growing areas
Autor/es:
PAPURA, D.; PIZZUOLO, P.; LUCERO, G.; LOPEZ PLANTEY, R.; BALLONI, A.; THIERRY, D.; RIQUELME, A.; BOITEAUX, J.
Lugar:
San Diego
Reunión:
Congreso; 50th Annual Meeting of the Society of Invertebrate Pathology; 2017
Institución organizadora:
International Society of Invertebrate Pathology
Resumen:
The appearance of new pests in a culture requires actions to prevent establishment, spreading internal or across international borders. The European grapevine moth Lobesia botrana was recently introduced in Argentina in 2010, when it was declared as quarantine pest. The current methods for controlling grape moth?s populations include conventional chemical ovicides or larvicides, but also alternative methods as mating disruption and Bt toxin. The use of entomopathogenic fungi as a control strategy of Lepidoptera is known from ancient times. Argentinean wine production is mainly concentrated in the western area of the country, next to the Cordillera de los Andes with a high specificity in the local environmental conditions. The aim of present work was to isolate and characterize natives entomopathogenic fungi from Argentinean grapevine environment, which could be propose as biological control agents against L. botrana. Soils from vineyards over 15 years old were sampled across 1200km on the Argentinean provinces of Mendoza, San Juan, Catamarca, La Rioja, Salta, Río Negro and Neuquén. A total of 32 entomopathogenic fungi strains were isolated from infected soil samples. These strains were characterized by dichotomous key and by molecular techniques using a partial sequence of nuclear protein-encoding gene, translation elongation factor 1-alpha. Three genders have been identified by morphological characterization and the molecular analysis confirmed more specifically that these are three species: Beauveria bassiana, Metarhizium robertsii and Paecilomyces lilacinus. The results provide a basis of the future physiological and pathogenic studies of these strains against L. botrana and their use as biological control.