Matrix effect for soluble carbohydrates in different grapevine tissues by capillary electrophoresis with indirect detection

MORENO D.; PICCOLI P.; BOTTINI R.; BERLI F.; SILVA MF.

Santiago

Simposio; - XXII Latin-American Symposium on Biotechnology, Biomedical, Biopharmaceutical, and Industrial Applications of Capillary Electrophoresis and Microchip Technology; 2016

Universidad de Chile

The qualitative and quantitative analyses of carbohydrates are useful for many purposes, mainly for food and beverages quality control, but also for the evaluation of plant physiology and in metabolic studies. Capillary electrophoresis (CE) has been proved to be a powerful green separation technique that can provide high resolution and efficiency with minimal sample preparation, even in complex grapevine tissues at different phenological stages. A background electrolyte (BGE) consisiteing of 7 mmol/L potassium sorbate, 0.50 mmol/L CTAB, 50 mmol/L NaOH and 10% (v/v) methanol at pH 12.80 was found to be optimal. Indirect detection was performed at 254 nm; hydrodynamic injection of 5 s at 30 mbar; -20 kV constant voltage. The source and levels of matrix effects in the determination of most abundant sugars in mature and young leaves, berries and roots of a grapevine by CE with indirect detection, at two different phenological times were evaluated. Sucrose was the sugar showing the least matrix effect, being fructose the analyte most affected. Concerning the different tissues, young leaves at both phenological stages showed the smallest matrix effect. These changes are the result of chemical and/or physical interactions of sugars and matrix components with the capillary wall. Therefore, quantification was carried out following the standard addition method.