Inoculation with Pseudomonas fluorescens RT6M10 and Azopirillum brasilense AZ39 increase growth, productivity and modify sugars in Arabidopsis thaliana

COHEN, A.; BOTTINI, R.; MORENO, D.; SILVA, M.F.; AGUERO, F.A.; PICCOLI, P.

Mendoza

Congreso; XXXIV Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2016

Sociedad de Biología de Cuyo

Plant growth promoting rhizobacteria (PGPR) are bacteria that can actively colonize plant roots and enhance plant growth. The use of beneficial PGPR as bioinoculants may enhance crop productivity in a sustainable and environmentally friendly manner. Pseudomonas sp. are a well-studied group of bacteria that promote plant growth. In a previous work, our group isolated Pseudomonas fluorescens Rt6M10 (Rt6M10) from roots and rhizosphere of grapevines from a commercial vineyard. Moreover, Azospirillum sp. is one of the best-studied PGPR. A. brasilense Az39 (Az39) is used as inoculant in Argentina. The aim of this study was to evaluate the effect of Rt6M10 and Az39 in plants of A. thaliana Col-0. Seeds of Col-0 were surface disinfected and placed in plastic pots containing peat moss and perlite. Then, pots were put into each tray and located in a growth chamber. The plants were watered to keep soil water status close to field capacity. Plants with two fully expanded leaves were inoculated with (1) Rt6M10, (2) Az39, (3) Rt6M10+Az39, and (4) Control. The treatments were arranged in a completely randomized design. After 60 days, leaf area (LA), photosynthetic and photoprotective pigments, relative leaf water content (RLWC) and the dry weight (DW) of rosettes were measured. Also, the percentage of plant survival and the total seeds production per plant (yield) were evaluated. The sugars were measured by capillary electrophoresis. Inoculation of Arabidopsis with Rt6M10 and/or Az39 improved the vegetative and reproductive success of the plant´s performance. The inoculation increased maltose levels (product of starch hydrolysis). Also, sucrose was determined in inoculates plants, while it weren´t detected in the control.