IBAM   22618
INSTITUTO DE BIOLOGIA AGRICOLA DE MENDOZA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Development of a high-performance liquid chromatography method based on a core-shell column approach for the rapid quantification of polyphenols in grape pomaces
Autor/es:
FONTANA, A.; ANTONIOLLI, A.; BOTTINI, R.
Lugar:
Honolulu
Reunión:
Congreso; 2015 International Congress of Pacific Basin Societies (Pacifichem 2015); 2015
Institución organizadora:
American Chemical Society
Resumen:
Grape pomace (GP) is the most abundant residue of winemaking industry consisting mainly of skins and seeds that remain after fermentation and maceration during wine elaboration. This byproduct contains high levels of polyphenols due to an incomplete extraction during winemaking process. The chemical characteristics of GP extracts (GPEs) are related to the content of phytochemicals with bioactive properties, and so it is of utmost significance to determine their composition aiming to the characterization of samples and likewise increase the value of the product. Considering the complexity of GPE matrix, LMW-PPs determination represents a noteworthy challenge in terms of achieving an efficient separation in shorter times.Core?shell particles are an alternative to improve HPLC separation efficiencies and speed without reducing particle size. Due to the particle is not fully porous, analytes spend less time diffusing through the column. This allows faster mass transfer, resulting in sharper and higher peaks equivalent to, or better than, sub-2 μm fully porous particles. That is, this new technology allows UHPLC performance on regular and cheaper HPLC instruments.The objective of this work was to develop a high throughput HPLC method using a core-shell chromatography approach coupled to multiple wavelength detection (MWD) for the determination of 20 LMW-PPs (phenolic acids, flavanols, flavonols, stilbenes and phenylethanol analogs) in GPEs. The separation of 20 LMW-PPs was achieved in less than 12 min by using a Kinetex C18 column (3.0 mm x 100 mm, 2.6 µm) with a gradient of ultrapure water (0.1 % formic acid) and acetonitrile, a temperature of 35 °C and a flow rate of 0.8 mL min-1. The maximum backpressure reached was 4750 psi, meaning the developed method is adequate for standard HPLC instruments. The optimized method was applied for the characterization of the major polyphenolic constituents of GPE coming from different grape varieties cultivated in Argentina.