Effect of wine grape pomace extract (WGPE) and wine grape pomace (WGP) on weight gain and adipose tissue inflammation in high-fat-fructose diet-induce metabolic syndrome

PERDICARO, D.J.; RODRIGUEZ LANZI, C.; ANTONIOLLI, A.; RAVOTTI, F.; FONTANA, A.; BOTTINI, R.; MIATELLO, R.M.; VAZQUEZ PRIETO, M.A.

Buenos Aires

Conferencia; VI International Conference on Polyphenols and Health (ICPH-6); 2013

School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina Institute of Molecular Biochemistry and Medicine, UBA-CONICET, Buenos Aires, Argentina

Polyphenols from grape has been found to exert beneficial effect on disorders associated with metabolic syndrome (MS). The aim of this study was to evaluate the effect of wine grape pomace extract (WGPE) and wine grape pomace (WGP) on weight gain, and metabolic alteration related with high fat-fructose diet-induce MS. The total phenolic content of WGPE and WGP were 196,2 and 41.6 mg of gallic acid equiv/g respectively. Catechin and epicatechin in WGPE were 2.14 and 3.29 mg/g, respectively. Other major polyphenols in WGPE included quercetin (1.54 mg/g) and trans-resveratrol (322 μg/g). Thirty male Wistar rats were fed for 6 w: control diet (C), high fat-fructose diet (20 % each w/w) (HFFD), HFFD supplemented with WGPE (100 and 300 mg/kg BW/d respectively), and HFFD plus WGP in a dose of 1 g/Kg BW/d. HFFD increased weight gain, systolic blood pressure, triglycerides and decreased HDL-cholesterol that were ameliorated/prevented by high doses of WGPE and WGP. HFFD also increased the area of adipocytes and parameters of adipose tissue inflammation that were partially reversed by high doses of WGPE and WGP. Consumption of food or food-rich in polyphenols may be useful in the prevention and/or amelioration of MS and MS-associated diseases.