Effect of grape pomace (GP) on weight gain and adipose inflammation in high-fat-fructose diet-induce metabolic syndrome

RAVOTTI, F.; RODRIGUEZ LANZI, C.; PERDICARO, D.J.; ANTONIOLLI, A.; FONTANA, A.; BOTTINI, R.; MIATELLO, R.M.; VAZQUEZ PRIETO, M.A.

Buenos Aires

Conferencia; VI International Conference on Polyphenols and Health (ICPH-6); 2013

School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina Institute of Molecular Biochemistry and Medicine, UBA-CONICET, Buenos Aires, Argentina

Western diet high in fat and sugars has been related with high incidence of cardiac arrhythmias. Food rich in polyphenols has been proposed to prevent cardiovascular disease. Our aim was evaluate if a high-fat-fructose diet (HFFD) increased arrhythmia in a model of injury by ischemia-reperfusion and the cardioprotective effect of dietary supplementation with wine grape pomace (WGP). Male Wistar rats were fed for 6 w: control diet (C), HFFD (20 % fat and 20% fructose w/w), and HFFD supplemented with WGP in a dose of 1 g/Kg/d (n=5 each group). The heart was extracted, reperfused with modified Krebs-Henseleit solution and performed in 5 m from preisquemia, 10 m of regional ischemia (by ligation of the anterior descending coronary artery) and 5 min of reperfusion. Incidence and severity of arrhythmias were analyzed with the scale of Curtis-Walker. HFFD showed high incidence and duration of severe arrhythmias group (4/5) compared with the C group that showed low incidence of severe arrhythmias (1/5). HFFD supplemented with WGP showed high incidence but low duration (4/5) restoring the normal electric activity. Our results suggest that HFFD predispose the occurrence of arrhythmias in the ischemia-reperfusion model. Interestingly, supplementation with WGP could attenuate this effect.