Matrix solid phase dispersion for the analysis of argentinean monovarietal olive oils

MONASTERIO, R.P.; SILVA, M.F.

Tenerife

Simposio; ITP 2013-20th International Symposium, Exhibit & Workshops on Electro- and Liquid Phase-separation Techniques; 2013

Olive (Olea europaea) is one of the oldest agricultural tree crops with remarkable cultural and economic importance. Virgin olive oil is obtained from mechanical pressing of ripe olive fruits without any further refining process. Olive oil is frequently associated with a low incidence of cardiovascular diseases as well as with antioxidant properties. These benefits have been associated to its well-balanced fatty acids composition, of which oleic acid is the main component and to the presence of minor biomolecules, such as vitamins, carotenoids, tocopherols, phenolic compounds, and other natural antioxidants, which may act, by different mechanisms, as an effective defense against reactive species. The absence of refined process allows the presence of minor components that give especial characteristics [1, 2]. Previous analysis by liquid chromatography or capillary electrophoresis, sample treatment is probably the most important step in olive oil. The complexity lies in the extraction of for example phenolic compounds without coextraction of lipids. Many approaches have been proposed to tackle this task. The most employed method to extract minor components from olive oil requires high amounts of organic solvents as well as relatively expensive instrumentation [3, 4]. In this work, a simple methodology using matrix solid phase dispersion (MSPD) as clean up for olive oil sample has been applied for the determination of phenolic compounds. MSPD was developed using Florisil as sorbent material, hexane as clean up and acidified methanol as elution solvent. The separation and determination were carried out HPLC with a multiple wavelength detector (MWD). This detector allow selected the best wavelength according to the analytes, achieving LOD and LOQ according to concentration present in the sample. The present methodology demonstrates that MSPD can be an excellent and green extraction technique for the separation and preconcentration of phenolic compounds present in olive oil.