IBAM   22618
INSTITUTO DE BIOLOGIA AGRICOLA DE MENDOZA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Transcriptional diversification in the STS gene family revealed by microarray analysis of the elicitation responses to MeJA and cyclodextrin
Autor/es:
CHIALVA, CS; CARBONELL BEJERANO, P; ALMAGRO, L; PEDRENO, MA; MARTINEZ-ZAPATER, JM; LIJAVETZKY, D
Lugar:
La Serena
Reunión:
Simposio; IX International Symposium on Grapevine Physiology and Biotechnology; 2013
Resumen:
Stilbenes are a small family of phenylpropanoids produced in a number of unrelated plant families including the Vitaceae. In the genus Vitis they are synthesized by several species, including V. vinifera L. Stilbenes are derivatives of the trans-resveratrol structure and are produced by plants and cell cultures as a response to biotic and abiotic stresses. Stilbene synthases (STS), which catalyze stilbene backbone biosynthesis, seem to have evolved from chalcone synthases (CHS) several times independently. To date, STSs have been cloned from peanut, pine, sorghum and grapevine, the only stilbene-producing fruiting-plant for which the entire genome has been sequenced. This reference genome (PN40024, Jaillon et al., 2007) has revealed an unusually large and closely related STS genes family. In this study we analyzed the gene expression profile corresponding to 16 different STS genes present in the custom GrapeGen Affymetrix GeneChip. The analysis was carried out using samples of grapevine cv Monastrell albino cell suspension cultures exposed to the elicitor effect of both cyclodextrin (CD) and methyljasmonate (MeJA), as well as the combined treatment. The results showed significant expression values for most of the STS studied genes, as well for the global gene expression, after the elicitation with the combined treatment. These findings are in correlation with a previous work (Lijavetzky D., 2008) in which the combined addition of MeJA and CD to V. vinifera cv. Monastrell albino cell cultures produced a synergistic effect on resveratrol accumulation and on two studied STS genes. Moreover, despite the high sequence similarity among those 16 genes, we observed clear differences between their expression patterns.