IBAM   22618
INSTITUTO DE BIOLOGIA AGRICOLA DE MENDOZA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
MOLECULARAND GENETIC ANALYSIS OFGRAPEVINE BERRY SIZE DETERMINATION. THE AINTEGUMENTA GENE FAMILY
Autor/es:
GRISSI C, MUÑOZ CJ , CHIALVA CS , GOMEZ TALQUENCA S , LIJAVETZKY D
Lugar:
La Serena
Reunión:
Congreso; The Ninth International Symposium on Grapevine, Physiology and Biotechnology; 2013
Resumen:
Fruit size is a highly important trait for most fruit and vegetable crops. This trait has been a main selection target from the beginning of the modern agriculture. Even though its determination is influenced by the cultural practices, several genes have been identified controlling fruit size and shape. The AINTEGUMENTA (ANT) gene is a member of the AP2/ERF family of transcription factor genes. In ovules of the ant mutant, integuments do not develop and megasporogenesis is blocked. Based on these results, it is believed that ANT regulates cell proliferation and organ growth by maintaining the meristematic competence of cells during organogenesis. On the other hand, the characterization in Arabidopsis and other plant species of seven closely related genes (ANT-like genes o AIL) also suggested their participation in specification of meristematic or division-competent states. Using the ANT and AIL known sequences we identified all the grapevine (Vitis vinifera) putative ortholog genes. After the aligned of their sequences we determined the phylogenetic relationship respect all the ANT and AIL sequences from Arabidopsis, rice and poplar. We designed qRT-PCR specific primers for all grapevine ANT/AIL genes and we analyzed their expression patterns in different tissues and stages of the plant with the aim of defining their putative role in the flower and fruit size determination