Anthocyanins profile variation within Vitis vinifera cv. Malbec clones

CJ MUÑOZ, S GOMEZ TALQUENCA, M FANZONE, A PEÑA,J M MARTINEZ-ZAPATER, D LIJAVETZKY

La Serena

Congreso; The Ninth International Symposium on Grapevine, Physiology and Biotechnology; 2013

The Ninth International Symposium on Grapevine, Physiology and Biotechnology

Abstrac Despite the total amount of anthocyanins in red grape cultivars varies significantly, the relative content of each anthocyanin (anthocyanin profile or fingerprint) remains constant, thus it is currently accepted that the anthocyanin fingerprint of red cultivars appears to be closely related to genetic characteristics. However, current research on the general fruit composition of several clones of Cabernet Sauvignon, Tempranillo and Pinot noir indicates that skin anthocyanin profile is quite similar in most studied clones. We analyzed 134 Malbec clones grown in the same vineyard in Agrelo (Mendoza). Clonal identity of the samples was verified by analyzing nine microsatellite markers. In order to characterize each of the 134 clones present in the studied collection we determined their anthocyanin profile by means of HPLC analysis. For each clone we determined the concentrations of delphinidin (Dp), cyanidin (Cy), petunidin (Pt), peonidin (Po) and malvidin (Mv) for their glycosylated, acetylated and cumarylated forms: Dp3Gl, Cy3Gl, Pt3Gl, Po3Gl, Mv3Gl, Dp3acGl, Cy3acGl, Pt3acGl, Po3acGl, Mv3acGl, Dp3cumGl, Cy3cumGl, Pt3cumGl, Po3cumGl y Mv3cumGl. The obtained profiles allowed clustering the clones in 10 contrasted groups. Principal Component Analysis showed that variation in Mv and Po concentration explained about 90% of the variation within the clones (Mv 63.5% & Po 25.5%, respectively). Based on this information we selected two contrasted groups of clones in order to perform microarray and qRT-PCR analyses with the aim of determine the molecular mechanisms underlining the phenotypic variation within the clones.