Development a Microsatellite Database for Identification of Olive Cultivars in Mendoza, Argentina?

R. TORRES; P. CORNEJO; V. BERTOLDI; M.S. FERRER; R. W. MASUELLI

Simposio; INTERNATIONAL SYMPOSIUM ON OLIVE GROWING; 2012

Olives were introduced inArgentina fromEurope over 400 years Ago. Several varieties are grown en Mendoza province and problems of misclassification of plants in orchards and nurseries there exists. This work was aimed to develop a cost effective protocol for fingerprinting olive germplasm using microsatellite (SSR) and generate a database in order to help the identification of olives trees in orchards and nurseries. Four highly informative polymorphic SSR (DCA3, DCA9, DCA11 and DCA16) were selected to fingeiprint olive cultivars. A protocol using a single dye-labeled forward .o-ñron primer with the M13 (-21) sequence was used in combination with the reverse specific SSR primers. PCR amplification were multiplexed and resolved in automated capillary genetic analyzer equipment. Profiles of the cultivars and a list of alleles with the sizes obtained by capillary electrophoresis are informed. Several genetic parameters were calculated for characterization of the loci analyzed. A database of SSR markers for ten of the more distributed olive varieties in Mendoza was developed.