IBAM   22618
INSTITUTO DE BIOLOGIA AGRICOLA DE MENDOZA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Effects of inoculations with Pseudomonas fluorescens, tungstate and diphenylamine in Arabidopsis thaliana plants
Autor/es:
ANA C. COHEN; CARLA SALVADORES; RUBÉN BOTTINI; PATRICIA N. PICCOLI
Lugar:
San Juan
Reunión:
Jornada; II Reunión Conjunta Sociedad de Biología de laRepública Argentina; 2011
Institución organizadora:
Sociedades de Biología de la República Argentina
Resumen:
Pseudomonas sp. are well-studied group of bacteria that promote plant growth (PGPR). In a previous study P. fluorescens was isolated from roots of Vitis vinifera (cv. malbec L.) and produce the plant hormone abscisic acid (ABA) in chemical-defined media. In addition, when incorporated diphenylamine (DPA, phytoene desaturase inhibitor) or sodium tungstate (W, an inhibitor of ABA-aldehyde oxidase,  with the consequent inhibition of synthesis of ABA in plants), production of ABA in culture media decreased. The aim of this study was to evaluate the effect of inoculation of P. fluorescens and the application of W and DPA in A. thaliana plants (C). Experiments were carried out on plates with MS modified with 12 h light at 22 °C. At 15 days the inhibitors were applied and 3 days later was inoculated with P. fluorescens (I). I stimulated leaf area, number of lateral roots, fresh and dry weight of both aereal part and roots. W increased the aereal part. However when applied W + I together, the effect was similar. W increased lateral root length, increasing the fresh and dry weight. W + I further increased the number of lateral roots. The application of DPA only altered the morphology of the root being more branched than C. The combination of DPA I was effective.