IBAM 22618
INSTITUTO DE BIOLOGIA AGRICOLA DE MENDOZA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
?Molecular bases of the post-zygotic barriers in interspecific crosses between wild potato species?.
Autor/es:
P. CORNEJO; R. W. MASUELLI
Reunión:
Congreso; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular.; 2011
Resumen:
To investigate the molecular bases of post-zygotic hybridization
barriers in tuber-bearing Solanums, two wild potato species,
Solanum commersonii and S. acaule were crossed in intra- and
interspecific genotypic combinations, and the transcriptome of
inmature seeds were analyzed by using the cDNA-AFLP technique.
From a total of 423 analyzed cDNA fragments, 107 were
differentially regulated between the intra and interspecific
combinations. Twenty one fragments were sequenced and five of
the cDNA-AFLP differential patterns were validated using RT-PCR
analysis. One of the selected fragments was used as probe for in situ
interspecific genotypic combinations, and the transcriptome of
inmature seeds were analyzed by using the cDNA-AFLP technique.
From a total of 423 analyzed cDNA fragments, 107 were
differentially regulated between the intra and interspecific
combinations. Twenty one fragments were sequenced and five of
the cDNA-AFLP differential patterns were validated using RT-PCR
analysis. One of the selected fragments was used as probe for in situ
and S. acaule were crossed in intra- and
interspecific genotypic combinations, and the transcriptome of
inmature seeds were analyzed by using the cDNA-AFLP technique.
From a total of 423 analyzed cDNA fragments, 107 were
differentially regulated between the intra and interspecific
combinations. Twenty one fragments were sequenced and five of
the cDNA-AFLP differential patterns were validated using RT-PCR
analysis. One of the selected fragments was used as probe for in situin situ
hybridization experiments. Finally, the amount of DNA in
endosperm nuclei of seeds was estimated based on the intensity of
the Hoechst staining in relation to the ploidy of the endosperm,
using ImageJ. Sequence analysis suggested a role for the
differentially expressed sequences in the vesicle transport,
cytokinesis, cell cycle and others. In-situ hybridization experiments
with a fragment encoding a vesicle transport protein revealed
expression of this gene in embryo and endosperm. The amount of
DNA was 1.5 times greater in interspecific cross compared to
intraspecific cross.
The results suggest and support the fact that the collapse of the
embryo and endosperm in interespecific crosses may be related to
the alteration in the vesicle transport machinery and cytokinesis.
