IBAM   22618
INSTITUTO DE BIOLOGIA AGRICOLA DE MENDOZA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
GENOMIC AND BIOCHEMICAL CHARACTERIZATION OF ANTHOCYANIN PROFILE VARIATION IN MALBEC GRAPEVINE CLONES
Autor/es:
MUÑOZ, CJ; GOMEZ TALQUENCA, S; PEÑA, A; MARTINEZ-ZAPATER JM; LIJAVETZKY, D
Lugar:
San Luis
Reunión:
Congreso; 47th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; 2011
Institución organizadora:
Sociedad Argentina de Investigación Bioquímica y Biología Molecular
Resumen:
The deep study of the variations observed within grapevine clones, for different phenotypic, agronomic and oenological traits may allow determining the particular potential of those clones in a collection or vineyard. Moreover, that information may lead to the identification of the molecular and genetic basis of the observed variation. In order to differentiate each of the 134 Malbec clones present in the studied collection we determined their anthocyanin profile by means of HPLC analysis. For each clone we determined the concentrations of delphinidin (Dp), cyanidin (Cy), petunidin (Pt), peonidin (Po) and malvidin (Mv) for their glycosylated, acetylated and cumarylated forms: Dp3Gl, Cy3Gl, Pt3Gl, Po3Gl, Mv3Gl, Dp3acGl, Cy3acGl, Pt3acGl, Po3acGl, Mv3acGl, Dp3cumGl, Cy3cumGl, Pt3cumGl, Po3cumGl y Mv3cumGl. Clonal identity of the samples was verified by analyzing nine microsatellite markers. The obtained profiles allowed clustering the clones in 10 contrasted groups. Principal Component Analysis (PCA) showed that variation in Mv and Po concentration explained about 90% of the variation within the clones: Mv 63.5% & Po 25.5%. Based on this information we selected two contrasted groups of clones in order to perform different transcriptomic, genetic and epigenetic analysis in order to determine the molecular mechanisms underlining the phenotypic variation within the clones.