Diphenylamine, Tungstate and Nitrogen modified ABA production by Pseudomonas fluorescens isolated from roots of Vitis vinifera

COHEN A C; BOTTINI R,; PICCOLI P N

Mendoza

Jornada; Reunión Científica Conjunta de la Soc. de Biología de Cuyo, Secyt y Post Grado UN de Cuyo, Secyt Fac. de Cs Médicas y Cs Agrarias UNCu-Dirección de Investigación, Ciencia y Técnica Ministerio de Salud Gobierno de Mendoza; 2010

Soc. de Biol de Cuyo, Secyt UNCuyo

Plant growth-promoting rhizobacteria (PGPR) colonize the root system, and have been studied as plant growth promoters for increasing agricultural production and as biocontrol agents against plant diseases. Pseudomonas fluorescens  has received particular attention throughout the global science because of their catabolic versatility, excellent root colonizing ability and their capacity to produce a wide range of enzymes and metabolites that favour the plant withstand under varied biotic and abiotic stress conditions. P. fluorescens was isolated from roots of Vitis vinifera (cv. malbec L.) and produce the plant hormone abscisic acid (ABA) in chemical-defined media. Tungstate (W) affects the formation of ABA from ABAaldehyde by impairing ABA-aldehyde oxidase. Diphenylamine (DPA) is an inhibitor of lycopene â-cyclase. This work studies the effect the W, DPA and Nitrogen (N) in the culture media and ABA production. The addition of 10 mM W, 10 mM DPA and 135 mM NH4Cl modified bacterial growth assessed as CFU ml-1 and ABA production.