Phitohormones production by indigenus bacteria isolated from roots of Vitis vinifera cv. Malbec

SALOMON M.V.; BOTTINI R.; PICCOLI P.

Mendoza

Otro; 28 Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2010

Sociedad de Biología de Cuyo

Although grape is the fruit of major economic importance of the world, few microbiological studies of soil and roots have been reportedfor the specie. One of the mechanisms by which bacteria can promote growth is by the production of phytohormones such as GAs, ABA, IAA and AJ. However, there are few studies about the use of bacteria to promote the grape wine growth. Previously,we isolated and characterized by 16S rRNA gene Sequencing analysisseveral bacteria from adjacent soil and vine roots of Vitis vinifera.cv. Malbec, among them, Pseudomonas fluorescens,Kokuria erytromyxa and Microbacterium imperiale. The aim of thisstudy was to evaluate the ability of these bacteria to produce plant  hormones in chemically defined medium, controlling DO530 (total biomass) and UFC.mL-1 (cell alive). At this time, by GC-EIMS analysis ABA and IAA characterization have not been yet reported. By using isotopically-labeled ABA and AIA the amounts produced by P. fluorescens, M. imperiale, K. erytromyxa were 7.1, 5.28 and 9.95 ng.mL-1 ABA, while the amounts of IAA were 28.5, 38.5 and 50.5 ng.mL-1, respectively. This is the first report of ABA and IAA production by indigenous bacteria isolated from grapevine roots, suggesting one mechanism by the bacteria for promote and benefit plant growth.