IBAM   22618
INSTITUTO DE BIOLOGIA AGRICOLA DE MENDOZA
Unidad Ejecutora - UE
artículos
Título:
Grape pomace extract supplementation activates FNDC5/irisin in muscle and promotes white adipose browning in rats fed a high-fat diet
Autor/es:
PERDICARO, DIAHANN J.; FONTANA, ARIEL R.; RODRIGUEZ LANZI, CECILIA; MUSCIA, VICTORIA; VAZQUEZ PRIETO, MARCELA A.; GAMBARTE TUDELA, JULIÁN; OTEIZA, PATRICIA I.; PERDICARO, DIAHANN J.; FONTANA, ARIEL R.; RODRIGUEZ LANZI, CECILIA; MUSCIA, VICTORIA; VAZQUEZ PRIETO, MARCELA A.; GAMBARTE TUDELA, JULIÁN; OTEIZA, PATRICIA I.
Revista:
Food & Function
Editorial:
Royal Society of Chemistry
Referencias:
Año: 2020 vol. 11 p. 1537 - 1546
ISSN:
2042-6496
Resumen:
Irisin is a myokine regulated by peroxisome proliferator-activated receptor gamma co-activator-1α(PGC-1α) in the exercising skeletal muscle and released into the bloodstream after cleavage of FNDC5. Circulating irisin can up-regulate UCP-1 expression in white adipose tissue (WAT) promoting the formation of brown-like adipocytes. The aim of this study was to evaluate if supplementation with a grape pomace extract (GPE) could activate the FNDC5/irisin pathway via PGC-1α in rats fed a high fat diet (HFD). For this purpose we characterized the activation of: i. the FNDC5/irisin pathway and AMPK in skeletal muscle and ii. proteins involved in the formation of brown-like cells in epididymal WAT (eWAT). Consumption of the GPE activated the FNDC5/irisin pathway, increased AMPK phosphorylation in skeletal muscle and enhanced irisin plasma levels. In eWAT, the GPE increased the level of proteins involved in WAT browning, i.e. PGC-1α, PPARγ, PRDM16 and UCP-1. The GPE also prevented HFD-induced adipocyte hypertrophy and systemic insulin resistance. Consistently, in L6 myotubes, (?)-epicatechin (EC), a flavonoid abundantin the GPE, prevented palmitate-mediated downregulation of FNDC5/irisin protein expression andsecretion, in part via PGC-1α activation. Consumption of the GPE, a winemaking residue rich in bioactive compounds, could be a beneficial strategy to counteract the adverse effects of Western style diets through the promotion of WAT browning.